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P. putida MnB1 and a non-Mn(II)-oxidizing MnB1 mutant streaked on plates containing Mn(II) (top) or ABTS (bottom). Mn(II) oxidation results in the formation of brown Mn oxides on colonies, while ABTS oxidation results in the formation of a diffusible purplish-green product. The mutant is incapable of oxidizing both substrates and remains opaque on plates.
