Table 5.
The optimized elution gradient for chromatographic separation.
| Time min | Mobile Phase A% 5 mM AFNH4 in H2O |
Mobile Phase B% MeOH |
|---|---|---|
| 0.0 | 95 | 5 |
| 6.0 | 50 | 50 |
| 10.0 | 5 | 95 |
| 15.0 | 5 | 95 |
| 15.1 | 95 | 5 |
| 18.0 | 95 | 5 |
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