FIG. 1.

Localization and physical map of the regions containing csaI or csaR and construction of disrupted versions of csaI and csaR. Arrows represent the location and orientation of the genes. (A) The location of the regions leading to elevated β-galactosidase activity was determined by measuring the effects of the indicated subclones of pLSP5-5 on β-galactosidase expression in the phzB::lacZ reporter strain 30-84Z. Letters indicate significant differences between values. (B) csaR and csaI loci from pZZG3 and insertional mutation of csaR and csaI. Restriction enzyme abbreviations: R, EcoRI; H, HindIII; Sp, SphI; V, EcoRV, Bg, BglII, P, PstI; S, SacI.