TABLE 3.
Complementation of P. aureofaciens 30-84 derivatives with csaR or csaR-csaI in trans
| Strainf | Genotype (host/plasmid) | Absorbance at 367 nmac | Protease activitybc | Colony morphologyd | Clumping phenotypee |
|---|---|---|---|---|---|
| 30-84(pLAFR3) | Wild type | 5.91 ± 0.29 A | + | Semidry and rough | − |
| 30-84R(pLAFR3) | phzR::Tn5lacZ | 0.03 ± 0.01 C | + | Semidry and rough | − |
| 30-84R2(pZZG11) | csaR::uidA-Gm/csaR+ | 5.98 ± 0.39 A | + | Semidry and rough | − |
| 30-84I/I2(pZZG11) | phzI::npt csaI::uidA-Gm/csaR+ | 0.04 ± 0.01 C | − | Semidry and rough | − |
| 30-84I/I2(pZZG3) | phzI::npt csaI::uidA-Gm/csaR+ csaI+ | 2.67 ± 0.11 B | − | Semidry and rough | − |
| 30-84I/R2(pZZG11) | phzI::npt csaR::uidA-Gm/csaR+ | 2.36 ± 0.20 B | + | Semidry and rough | − |
| 30-84I2/R(pZZG3) | csaI::uidA-Gm phzR:Tn5lacZ/csaR+csaI+ | 2.57 ± 0.20 B | + | Semidry and rough | − |
| 30-84R/R2(pZZG11) | phzR::Tn5lacZ, csaR::uidA-Gm/csaR+ | 2.42 ± 0.19 B | − | Semidry and rough | − |
| 30-84.gacA(pZZG3) | gacA::npt/csaR+csaI+ | 0.03 ± 0.00 C | − | Shiny and mucoid | + |
Phenazines were extracted from supernatants of 24-h-old bacterial cultures grown in PPM-D and quantified by measuring absorbance at 367 nm.
Exoprotease activity in supernatants of 24-h-old broth cultures was determined by a modified Lowry method using casein as the substrate.
All values are means ± standard deviations of two experiments, with three replicates per experiment. Values followed by the same letter in a column are not significantly different (P = 0.05).
Bacterial strains were cultured on KMB agar, and 2 days later the colonies were examined for roughness and mucoidy.
Bacterial strains were cultured on KMB broth for 24 h, and the cultures were stained with Congo red and observed for the presence of the clumping phenotype.
The presence of the vector pLAFR3 alone had no effect on any of the strains listed (data not shown).