TABLE 4.
Bacterial populations of 30-84 and its derivatives recovered from the wheat rhizospherea
Strain | Genotype | Log CFU/g of rootsb
|
|
---|---|---|---|
Pasteurized soil | Natural soil | ||
30-84 | Wild type | 7.68 ± 0.27 A | 7.14 ± 0.26 A |
30-84I | phzI::npt | 7.60 ± 0.25 A | 6.42 ± 0.23 B |
30-84R | phzR::Tn5lacZ | 7.54 ± 0.26 A | 6.32 ± 0.23 B |
30-84I2 | csaI::uidA-Gm | 7.54 ± 0.24 A | 6.20 ± 0.29 B |
30-84R2 | csaR::uidA-Gm | 7.63 ± 0.21 A | 6.89 ± 0.28 A |
30-84I/I2 | phzI::npt csaI::uidA-Gm | 6.98 ± 0.29 B | 5.44 ± 0.28 C |
30-84R/R2 | phzR::Tn5lacZ csaR::uidA-Gm | 6.94 ± 0.30 B | 5.46 ± 0.23 C |
Pregerminated wheat seeds were treated with a bacterial suspension and sown in potting mix containing pasteurized or natural soil contained in plastic cones. The cones were placed in a growth chamber. Twenty days after emergence, root samples were collected and bacteria were isolated.
All values are means ± standard deviations of two experiments, with four replicates per experiment. Values followed by the same letter in each column are not significantly different (P = 0.05).