Table 1.
List of compounds revealed by LC-MS analysis of Moringa extract.
| No. | Retention Time (RT) (min) | Formula | Calculated Mass (Da) | Theoretical Mass (Da) | Mass Error (ppm) | MSE Fragmentation | Identification | Ref. |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.6 | C7H6O4 | 151.17 | 154.0266 | 3.2 | 153.0215[M − H]−, 135.0211[M-H-H2O]−, 89.0340[M-H-H2O-HCOOH]− | 3,4-Dihydroxy-benzoic acid | [20] |
| 2 | 1.8 | C7H12O6 | 191.1683 | 192.0634 | −1.10 | 191.0542[M − H]−, 173.0432[M-H-H2O]−, 145.0516[M-H-HCOOH]−, 137.0232[M-H-3H2O]−, 127.0401[M-H-H2O-HCOOH]− | Quinic acid | [21] |
| 3 | 3.1 | C9H8O3 | 163.19 | 164.0474 | 0.3 | 165.0544[M + H]+, 147.0444[M + H-H2O]+, 119.0483[M + H-HCOOH]+ | o-Coumaric acid | [22] |
| 4 | 6.6 | C17H20O9 | 369.26 | 368.1107 | −1.5 | 367.1029[M − H]−, 336.0902[M-H-OCH3 ]−, 295.1124[M-H-4H2O]−, 243.0591[M-H-CH3-C6H5O2]−, 189.0549[M-H-CH3-C9H7O3 ]−, 178.0346[M-H-C8H13O5] | Methyl-3-caffeoylquinate | [23] |
| 5 | 7.7 | C21H20O12 | 463.31 | 464.0955 | −3.4 | 463.0866[M − H]−, 318.0758[M-H-2H2O-C6H5O2]−, 178.0513[M-H-C15H9O6]−, 159.0379[M-H-Glu-C6H4O3]− | Isoquercetin | [24] |
| 6 | 7.9 | C21H20O12 | 464.0955 | 464.0949 | −1.3 | 465.1022[M + H]+, 285.0485[M + H-Glu]+, 231.0678[M + H-Glu-3H2O]+, 149.0150[M + H-Glu-C7H4O3]+, 152.0154[M + H-Glu-C8H5O2]+ | Hyperoside | [25] |
| 7 | 12.4 | C10H12O2 | 164.0837 | 164.0831 | −2.8 | 209.1118[M + HCOO]−, 122.0453[M-H-C3H5]−, 105.0495[M-H-OCH3-C2H3]− | Eugenol | [24] |
| 8 | 13.1 | C16H18O9 | 356.38 | 354.0951 | 0.1 | 353.0878[M − H]−, 253.1035[M-H-3H2O-HCOOH]−, 190.0182[M-H-3H2O-C6H5O2]−, 144.0302[M-H-H2O-C7H11O6]−, 125.0251[M-H-H2O-HCOOH-C9H8O3]− | Chlorogenic acid | [23] |
| 9 | 15.1 | C12H16O4 | 224.1038 | 224.1049 | −4.8 | 223.0965[M − H]−, 205.1027[M-H-H2O]−, 135.0421[M-H-C4H8O2]−, 123.0964[M-H-C4H4O3]−, 87.0295[M-H-C8H8O2] | 3-Butylidene-4,5,6,7 -tetrahydro-6,7-dihydroxy-1(3H)-isobenzofuranone | [26] |
| 10 | 15.3 | C12H14O4 | 222.0883 | 222.0892 | −3.9 | 221.0811[M − H]−, 160.0546[M-H-OC2H5]−, 119.0282[M-H-C3H5O2 -C2H5]−, | Diethyl phthalate | [23] |
| 11 | 20.9 | C18H30O2 | 278.2237 | 278.2246 | −3.0 | 277.2165[M − H]−, 182.1234[M-H-C7H11]−, 168.1230[M-H-C8H13]−, 110.0795[M-H-C11H17-H2O]− | Linolenic acid | [21] |
| 12 | 23.7 | C20H26O9 | 410.1573 | 410.1577 | −1.0 | 409.1504[M − H]−, 336.0817[M-H-C4H9O]−, 251.1394[M-H-2H2O-C7H6O2]−, 202.0639[M-H-C3H7-C9H7O3]−, 134.0437[M-H-C12H19O7]− | 5-O-Caffeoylquinic acid butyl ester | [20] |
| 13 | 26.9 | C18H34O2 | 282.2558 | 282.2559 | −0.4 | 283.2631[M + H]+, 97.1020[M + H-C5H11-C6H11O2]+, 86.1024[M + H-C12H21O2]+, 72.0876[M + H-C13H23O2]+ | Oleic acid | [21] |
| 14 | 39.2 | C9H16O4 | 188.1045 | 188.1049 | 2.1 | 187.0965[M − H]−, 141.1105[M-H-HCOOH]−, 123.0957[M-H-H2O-HCOOH]−, 112.0644[M-H-H2O-C3H5O]− | Azelaic acid | [20] |