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. 2022 Jul 13;14(14):3410. doi: 10.3390/cancers14143410

Figure 8.

Figure 8

C8orf76 activates SLC7A11 at the transcriptional level through directly binding to the promoter of SLC7A11. (A,B) SLC7A11 was analyzed using qPCR in HCC cells stably knocking down or overexpression of C8orf76 (n = 3). (C,D) The protein expression of SLC7A11 was analyzed by Western blot after C8orf76 knockdown or overexpression in HCC cells. (E) The localization of C8orf76 was viewed by laser scanning confocal microscope in both HA22T and HCCLM3 cells. Scale bars represent 20 μm. (F) Two potential C8orf76 DNA−binding motifs in the promoter region of SLC7A11. (G) CHIP-qPCR analysis was performed to determine the relation between C8orf76 and the promoter of SLC7A11. (H) The relative activities of the SLC7A11 promoter after transfection of C8orf76 and vector. (I) The relative activities of the SLC7A11 promoter and the mutant promoter after transfection of C8orf76 and vector. (J) C8orf76 and SLC7A11 protein expression in subcutaneous xenografts detected by immunohistochemistry. Scale bars represent 50 μm. (K,L) C8orf76 mRNA level is positively correlated with SLC7A11 mRNA levels in 37 patients and the TCGA dataset. Data were presented as mean +SD. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant. WT, wild type; M1, mutated C8orf76 recognition site 1; M2, mutated C8orf76 recognition site 2.