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. 2022 Jul 21;14(14):3538. doi: 10.3390/cancers14143538

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Construction of the structural domains of two shRNAs. (b) Analysis results of the effect of NNMT knockdown as determined by Western blot. (c) Evaluation of cell proliferation using WST-1 assay. (d) Microscopic observation of cell growth. (e) Scratch assay comparing the migration of U251 glioma cells after knockdown of NNMT. (f) Scratch assay comparing the migration of U87 glioma cells after knockdown of NNMT. (g) Invasion assay comparing the variations in the invasive ability of U251 and U87 glioma cells after knockdown of NNMT. Values are expressed as means ± SEM, ** p < 0.01, compared with NC.