Figure 5.

Knockdown comparison of rationally designed formulation vs. runs with neighboring conditions. The rationally selected LbL parameters of pH 6, ionic strength of 150 mM, PBAE concentration of 1.0 mg/mL, and siRNA concentration of 30 μg/mL were found to achieve in vitro knockdown of 47%. Run 6 (37% knockdown) and Run 10 (2% knockdown) from the fractional factorial design both neighbor these conditions with a single solution condition parameter varied. Colors denote high (red), moderate (yellow), and low (blue) values of parameters within the experimental range of the design. Error bars represent standard deviation.