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. Author manuscript; available in PMC: 2022 Jul 26.
Published in final edited form as: Nature. 2021 Sep 22;597(7878):693–697. doi: 10.1038/s41586-021-03933-1

Extended Data Figure 4. Chromatin accessibility precedes gene expression in branch 1- specific genes.

Extended Data Figure 4.

a, Heatmap depicting gene expression, Promoter and distal elements accessibility throughout branch 1 pseudotime for loci +/− 500kb around six branch 1 - specific genes. Distal elements are selected based on the relevance for classifying branch 1 cells. For each gene, row 1 shows gene expression, row 2 promoter accessibility, row 3 aggregated accessibility and the remaining rows are distal branch 1 classifying elements. Each trace has been smoothed using the lowess function in R.

b, Gene expression, Promoter and Aggregated accessibility throughout branch 1 pseudotime for 10 branch 1 - specific genes.

c, Only postmitotic cells are classified as branch neurons by supervised classification methods.

c-i) Classification of E13 cells into mitotic or postmitotic cells based on cell cycle RNA score.

c-ii) Classification of E13 cells into mitotic or branch 1,2,3 lineages based on chromatin accessibility.