TABLE 1.
Lipid composition of P. putida DOT-T1E growing in the absence and in the presence of 4HBAa
| Fatty acid | % of total lipid with 4HBA concn (g/liter) of:
|
|||
|---|---|---|---|---|
| 0 | 6 | 12 | 15 | |
| C14:0 | 0.95 | 1.15 | 0.47 | 0.36 |
| C15:0 | 0.12 | 0.04 | 0.06 | 0.05 |
| C16:1,9cis | 9.8 | 15.2 | 12.2 | 14.8 |
| C16:1,9trans | 1.4 | 8.7 | 25.1 | 23.5 |
| C16:0 | 42.8 | 42.7 | 40.9 | 39.9 |
| C17:cyclopropane | 26.7 | 16.1 | 4.5 | 1.6 |
| C17:0 | 0.16 | 0.08 | 0.07 | 0.07 |
| C18:1,11cis vaccenic | 17.0 | 14.0 | 13.1 | 14.6 |
| C18:1,11trans vaccenic | 0.11 | 0.80 | 4.9 | 4.0 |
| C18:0 | 0.92 | 1.1 | 1.1 | 2.2 |
| saturated to unsat | 1.6 | 1.2 | 0.7 | 0.73 |
| cis to trans | 17.8 | 3.1 | 0.84 | 1.1 |
a
One hundred milliliters of glucose-supplemented M9 minimal medium with the indicated concentration of 4HBA was inoculated with 1 ml of an overnight culture cultivated with glucose. After incubation for 24 h, 30 ml was harvested and washed with glucose-free M9 minimal medium. Lipids were extracted, transesterified, separated by gas chromatography, and identified by mass spectrometry as previously described (37). Experiments were repeated three times, and each sample was analyzed in duplicate. Standard deviations were less than 10% of the average given value.