TABLE 2.
Enzyme activities in extracts of G. nitida LE31 grown on 3-EP, 3-MP, and acetate as sole sources of carbon
| Enzyme | Product measured | Sp act (mUa/mg of protein) for the following growth substrate:
|
||
|---|---|---|---|---|
| 3-EP | 3-MP | Acetate | ||
| Isocitrate dehydrogenase | NADH | 7 | 3 | 49 |
| Levulinic aldehyde dehydrogenase | NAD | 28 | 24 | 11 |
| Methylsuccinic aldehyde dehydrogenase | NAD | <1 | <1 | 1 |
| 2-Methylglutaric aldehyde dehydrogenase | NAD | 2 | <1 | <1 |
| Formamidase | NH3 | 24 | 19 | <1 |
a
One unit is defined as the amount of enzyme that produces 1 μmol of product per min.