Table 1.
List of antibodies used for Western blot analysis and immunohistochemistry (IHC)
| Peptide/ Protein target | Name of antibody | Name of individual providing the antibody | Species raised (Monoclonal or Polyclonal) | Research Resource Identifier (RRID) | Dilution used |
|---|---|---|---|---|---|
| Neuregulin-1β (Nrg1) | Anti-Neuregulin-1β (Nrg1) | Santa Cruz, CA, USA | Rabbit polyclonal | AB_675755 |
1:1000 (WB) 1:200 (IHC) |
| ErbB3 receptor | Anti-ErbB3 receptors | Millipore, USA | Mouse monoclonal | AB_309713 |
1:1000 (WB) 1:200 (IHC) |
| pErk1/2 | Anti-pErk1/2 | Cell Signaling, Beverly, MA, USA | Mouse monoclonal | AB_2297442 | 1:1000 |
| Total Erk1/2 | Anti-Total Erk1/2 | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_331775 | 1:1000 |
| pAkt | Anti-pAkt | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_329825 | 1:1000 |
| Total Akt | Anti-Total Akt | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_329827 | 1:1000 |
| pPI3K | Anti-pPI3K | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_659940 | 1:1000 |
| Total PI3K | Anti-Total PI3K | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_659889 | 1:1000 |
| FSH-R | Anti-FSH-R | Abcam, Waltham, MA USA | Sheep polyclonal | AB_2294259 | 1:1000 |
|
Aromatase (Cyp19A1) |
Anti- Aromatase (Cyp19A1) |
Abcam, Waltham, MA USA | Rabbit monoclonal | AB_444718 | 1:1000 |
| β-Actin | Anti-Beta-Actin | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_330288 | 1:1000 |