Figure 6.

The net Cd²⁺ fluxes, metal concentration, Cd amounts, Cd bio-concentration factor (BCF) of WT and transgenic lines. (A,B) Net Cd²⁺ fluxes and the mean fluxes of Cd²⁺ in the roots, (C–E) Cd concentrations and amounts in different tissues and bio-concentration factor of WT and transgenic lines (PcNRAMP1-OE5, PcNRAMP1-OE8, PcNRAMP1-OE9) of P. × canescens treated with either 0 (−Cd) or 100 (+Cd) μM CdCl₂ for 2 weeks. Data indicate means ± SE (n = 4). Different letters on the bars indicate significant differences between the treatments. p-values of the two-way ANOVAs of genotype (G), Cd and their interactions (G × Cd) are also indicated. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant.