Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 8;23(14):7593. doi: 10.3390/ijms23147593

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Transport activities of Cd of mutated PcNRAMP1 in yeast. (A) Sequence alignment of different transmembrane segments of PcNRAMP1 and its homologous proteins in P. trichocarpa (Pt), A.thaliana (At), and O.sativa (Os). TMS: transmembrane segments. Dashed line in panel (A): selected mutation sites. (B) The growth status of ∆ycf1 transformed with empty vector, PcNRAMP1, and the mutated PcNRAMP1 (PcNRAMP1^D61A, PcNRAMP1^G63A, PcNRAMP1^M236A and PcNRAMP1^P405A) under 0 (−Cd) or 30 μM (+Cd) CdCl₂ for 4 days. (C) Cd concentration in yeast cells transformed with mutated PcNRAMP1. Bars indicate means ± SE (n = 4). Different letters on the bars indicate significant differences. The p-value of the one-way ANOVA of genotypes (G) is also indicated. *: p < 0.05.