Figure 1.

Accumulation of PVY RNA (A) and PVX RNA (B) (measured using RT-qPCR) in systemically infected leaves of potato plants cv. Indigo untreated or pre-treated with PVY dsRNA over a 7–21 dpi time periods as shown. PVY RNA and PVX RNA expression levels were normalized to those of internal controls, StEF-1α and StCox. Statistical analysis was performed on four independent biological replicates. Data are mean ± SD. Each replicate was composed of samples from three plants pulled pooled together (two leaves per plant). ANOVA and Tukey’s HSD post hoc tests were performed for RT-qPCR data. The different letters (a, b) indicate significantly different values (p < 0.001) in PVY RNA accumulation between dsRNA-treated and -untreated plants in a given time point. NS, not significant.