Figure 2.

Production of PVY-specific siRNAs induced by exogenous PVY dsRNA application in mock or systemically infected PVY plants (7 and 14 days post treatment as shown). Mock or PVY infected plants not treated with dsRNAs were used as controls. Ethidium bromide (EtBr) stained 5S rRNA+tRNA samples are shown as loading controls. Positions of 26 and 21 nt-size markers are indicated. Uncropped image is presented in Supplementary Figure S1. Similar results were obtained using four independent samples described in Figure 1 experiment.