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. 2022 Jul 18;23(14):7915. doi: 10.3390/ijms23147915

Figure 3.

Figure 3

Modulation of PTI-based defence responses in potato plants cv. Indigo to PVY or PVX infection via application of PVY dsRNA. (A–E) Expression patterns of PTI-related genes, StWRKY29 (A), StRbohD (B), StPR-1b (C), StEDS5 (D), and StSERK3/BAK1 (E) analysed by RT-qPCR in systemically or mock infected leaves at 7 days post treatment. StWRKY29, WRKY transcription factor 29; StRbohD, respiratory burst oxidase homolog D; StPR-1b, pathogenesis-related gene 1b; StEDS5, enhanced disease susceptibility 5; SERK3/BAK1, somatic embryogenesis receptor kinase 3 encoding brassinosteroid-insensitive 1-associated receptor kinase 1; PR-1b, pathogenesis-related gene 1b. mRNA expression levels were normalized to those of internal controls, StEF-1α and StCox. Statistical analysis was performed on four independent biological replicates. Data are mean ± SD. Each replicate was composed of samples from three plants pulled pooled together (two leaves per plant). Analysis of variance and Tukey’s HSD post hoc tests were performed on the data obtained. The different letters (a, b, c) indicate significantly different values (p < 0.001).