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. 2022 Jul 18;23(14):7915. doi: 10.3390/ijms23147915

Figure 5.

Figure 5

Modulation of poly(ADP-ribose) metabolism in potato plants cv. Indigo by PVY dsRNA in the presence or absence of PVY or PVX infection. (A,B) The expression patterns of genes encoding poly(ADP-ribose) polymerase (StPARP1, (A)) and poly(ADP-ribose) glycohydrolase (StPARG, (B)), analysed by RT-qPCR in systemically or mock infected leaves of Indigo plants at 7 days post treatment, as shown. mRNA expression levels were normalized to those of internal controls, StEF-1α and StCox. Statistical analysis was performed on four independent biological replicates. Data are mean ±SD. Each replicate was composed of samples from three plants pulled pooled together (two leaves per plant). (C) Accumulation of PARylated proteins measured by ELISA using rabbit anti-PAR polyclonal antibody, in systemically infected or mock-inoculated plants at 7 days post treatment. Statistical analysis was performed on four independent biological replicates. Analysis of variance and Tukey’s HSD post hoc tests were performed on the data. The different letters (a, b, c) indicate significantly different values (p < 0.001).