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. 2022 Jul 11;12(7):636. doi: 10.3390/metabo12070636

Figure 6.

Figure 6

TXNIP promoter sequence (+162/+218) and the effect of taurine on TXNIP promoter activity by employing site-directed mutagenesis. (A) Human TXNIP promoter sequences between +162 and +218 are shown. The sequence of putative Tst-1 and Ets-1 response sequences and their mutants are shown, respectively. (B,C) Caco-2 cells were transfected with a reporter vector containing the partial promoter region of TXNIP (−39/+256; wild type, Tst-1 mutant, Ets-1 mutant) and then replaced with the medium containing 100 mM of taurine. After 24 h, the cells were subjected to the luciferase assay. Results are expressed as relative values with the control value of mock as 1. Each value is the mean ± S.E. (n = 4) and the abcd values indicated by different characters are significantly different from each other and the abcd values indicated by the same characters are not significantly different (Tukey’s test; p < 0.05).