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. 2022 Jun 24;14(7):1336. doi: 10.3390/pharmaceutics14071336

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Synergistic effect of PC-NE and GEM on the growth of MCF-7 cells. (A) The viability data of MCF-7 cells from the MTT assay are exhibited for PC-NE:GEM, PC-NE, and GEM. Cells were treated with 0–1.2% (v/v) PC-NE or 0–1.2% (v/v) + 0–1.2 µM (PC-NE:GEM) or 0–50 µM GEM for 24 h. Data are shown as mean ± SEM (n = 3). (B) PC-NE synergized with GEM; fraction affected (Fa) and CI values and total doses for PC-NE and GEM combination (constant ratio 1:1) in MCF-7 cells. The CI, isobologram, and Fa values were estimated using CompuSyn software. (C) The combination index (CI) plot and isobologram based on the data obtained from the MTT assay. (D) Morphological changes in MCF-7 cells in response to GEM, PC-NE, and PC-NE:GEM treatment for 24 h. Cells were treated with the following concentrations: 0.3% (v/v) PC-NE, 0.3% (v/v) + 0.3 μM PC-NE(GEM), and 0.3 μM GEM. Magnification: 200×.

Synergistic effect of PC-NE and GEM on the growth of MCF-7 cells. (A) The viability data of MCF-7 cells from the MTT assay are exhibited for PC-NE:GEM, PC-NE, and GEM. Cells were treated with 0–1.2% (v/v) PC-NE or 0–1.2% (v/v) + 0–1.2 µM (PC-NE:GEM) or 0–50 µM GEM for 24 h. Data are shown as mean ± SEM (n = 3). (B) PC-NE synergized with GEM; fraction affected (Fa) and CI values and total doses for PC-NE and GEM combination (constant ratio 1:1) in MCF-7 cells. The CI, isobologram, and Fa values were estimated using CompuSyn software. (C) The combination index (CI) plot and isobologram based on the data obtained from the MTT assay. (D) Morphological changes in MCF-7 cells in response to GEM, PC-NE, and PC-NE:GEM treatment for 24 h. Cells were treated with the following concentrations: 0.3% (v/v) PC-NE, 0.3% (v/v) + 0.3 μM PC-NE(GEM), and 0.3 μM GEM. Magnification: 200×.