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. 2022 Jun 24;14(7):1336. doi: 10.3390/pharmaceutics14071336

Figure 8.

Figure 8

Effect of PC-NE and PC-NE:GEM on apoptosis of MCF-7 and Hep-G2 cells, as was estimated with Annexin V-FITC-PI staining and flow cytometry. (A) MCF-7 control cells; (B) MCF-7 cells treated with 0.3 μM GEM; (C) MCF-7 cells treated with 0.3% v/v PC-NE; (D) MCF-7 cells treated with PC-NE:GEM (0.3% (v/v) + 0.3 μM); (E) Hep-G2 control cells; (F) Hep-G2 cells treated with 0.5 μM GEM; (G) Hep-G2 cells treated with 0.5% (v/v) PC-NE; (H) Hep-G2 cells treated with PC-NE:GEM (0.5% (v/v) + 0.5 μM). (I) Percentages of apoptosis (early plus late) in MCF-7 and Hep-G2 cells. The statistical differences were determined by independent sample t-test, one-way ANOVA, and Tukey’s post hoc test. The data are expressed as mean ± SEM (n = 3); *** p  <  0.001; ns = not significant.