ANOVA and post-hoc analysis of the fALFF at the global and the nodal level, for wavelet scale 3. (a) Global level ANOVA and post-hoc pairwise analysis. Eyes closed states generally had higher fALFF, and fALFF was only affected by the eyes open/closed factor, independently of the light on/off factor or the interaction between the two. (b) Median variance of the fALFF of all four conditions. (c) Permutation tests at the nodal level for the main effect of eyes open/closed. Significant effects are visible in the secondary but not the primary visual cortex. There were no statistically significant nodal tests for the effect of light on/off or the interaction. Error bars show mean±SD. *P<0.05, **P<0.01, and ***P<0.001.