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. 2022 Jul 20;23(14):7992. doi: 10.3390/ijms23147992

Figure 3.

Figure 3

miR-143-3p inhibits tau phosphorylation. (A) SH-SY5Y cells were transfected with miR-143-3p or NC for 48 h, and the levels of tau phosphorylated at Thr231, Ser262, and Ser396, total tau, and DAPK1 were analyzed by immunoblotting. (B–E) Relative quantification was performed using ImageJ software, and the results are presented as a histogram. (F) SK-N-BE(2) cells were transfected with miR-143-3p or NC for 48 h, and the levels of tau phosphorylated at Thr231, Ser262, and Ser396, total tau, and DAPK1 were analyzed by immunoblotting. (G–J) Relative quantification was performed using ImageJ software, and the results are presented as a histogram. The data (circles and squares) are presented as the mean ± SD of three independent experiments (** p < 0.01, *** p < 0.001, N.S., not significant).