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. 2022 Jul 20;23(14):7992. doi: 10.3390/ijms23147992

Figure 5.

Figure 5

miR-143-3p promotes neurite outgrowth and microtubule assembly. (A) SH-SY5Y cells were transfected with miR-143-3p or NC for 24 h prior to cotreatment with RA. The levels of Tuj1 were detected by an immunofluorescence assay. Nuclei were stained with DAPI. Scale bars: 100 μm. (B) The average neurite length per cell was quantified in ten random images by ImageJ software and is presented as a histogram. (C,D) SH-SY5Y cells were transfected with miR-143-3p or NC for 48 h or treated with paclitaxel or nocodazole for 12 h and then lysed with hypotonic buffer. Equal amounts of cytosolic (S, soluble) and cytoskeletal (P, polymerized) proteins were separated by SDS-PAGE, and the levels of tubulin were analyzed by immunoblotting. Relative quantification was performed using ImageJ software, and the results are presented as a histogram. The tubulin expression level in the cytosolic/soluble fraction obtained from each treatment group was arbitrarily defined as ‘1’. The data (circles and squares) are presented as the mean ± SD of three independent experiments (** p < 0.01, *** p < 0.001).