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. 2022 Jul 20;23(14):7992. doi: 10.3390/ijms23147992

Figure 6.

Figure 6

miR-143-3p attenuates APP phosphorylation and inhibits the secretion of Aβ40 and Aβ42. (A–I) SH-SY5Y (A–C), SK-N-BE(2) (D–F), and SH-SY5Y APP (G–I) cells were transfected with miR-143-3p or NC for 48 h, and the levels of APP phosphorylated at Thr668, total APP, and DAPK1 were analyzed by immunoblotting. (J,K) SH-SY5Y APP cells were transfected with miR-143-3p or NC for 48 h, and the levels of human Aβ40 (J) and Aβ42 (K) in cell culture supernatants were determined by a solid-phase sandwich ELISA. Relative quantification was performed using ImageJ software, and the results are presented as a histogram. The data (circles and squares) are presented as the mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001, N.S., not significant).