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. 2022 Jul 20;23(14):7992. doi: 10.3390/ijms23147992

Figure 7.

Figure 7

Restoring DAPK1 antagonizes the effects of miR-143-3p in decreasing tau phosphorylation. (A) SH-SY5Y cells were transfected with NC, miR-143-3p alone or miR-143-3p together with a plasmid encoding human DAPK1 for 48 h, and the levels of tau phosphorylated at Thr231, Ser262 and Ser396, total tau, and DAPK1 were analyzed by immunoblotting. (B–E) Relative quantification was performed using ImageJ software, and the results are presented as a histogram. The data (circles, squares and triangles) are presented as the mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001, N.S., not significant).