Skip to main content
. 2022 Jul 20;23(14):7992. doi: 10.3390/ijms23147992

Figure 8.

Figure 8

Restoring DAPK1 antagonizes the effects of miR-143-3p in reducing APP phosphorylation and Aβ secretion. (A–C) SH-SY5Y APP cells were transfected with NC, miR-143-3p alone or miR-143-3p together with a plasmid encoding human DAPK1 for 48 h, and the levels of APP phosphorylated at Thr668, total APP, and DAPK1 were analyzed by immunoblotting. (D,E) SH-SY5Y APP cells were transfected with NC, miR-143-3p alone or miR-143-3p together with a plasmid encoding human DAPK1 for 48 h, and the levels of human Aβ40 (D) and Aβ42 (D) in cell culture supernatants were determined by solid-phase sandwich ELISA. Relative quantification was performed using ImageJ software, and the results are presented as a histogram. The data (circles, squares and triangles) are presented as the mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001, N.S., not significant).