Table 1.

PCR thermal cycles for SSU, ITS, LSU, RPB2 and TEF1α amplification.

Step	SSU			ITS, LSU, RPB2			TEF1α
	Temperature	Time	Cycles	Temperature	Time	Cycles	Temperature	Time	Cycles
Initial Denaturation	98 °C	2 min	1	98 °C	2 min	1	98 °C	2 min	1
Denaturation	98 °C	10 s	35	98 °C	10 s	35	98 °C	10 s	35
Annealing	47 °C	10 s		56 °C	10 s		61.7 °C	10 s
Extension	72 °C	10 s		72 °C	10 s		72 °C	10 s
Final Extension	72 °C	5 min	1	72 °C	5 min	1	72 °C	5 min	1
Hold	4 °C	-	-	4 °C	-	-	4 °C	-	-