Figure 3.

Effects of NaCl (200 mM) stress on steady-state Na⁺ flow in different regions of root tips of transgenic roots (TRs) and adventitious roots (ARs) of IbIPUT1. (A) Net Na⁺ flux measurement via NMT in adventitious roots (ARs) and IbIPUT1-transgenic roots (TRs). The steady-state Na⁺ flux was measured from the meristem (300, 400 and 500 µm from the tip), elongation (1.5, 2.0 and 2.5 mm from the tip) and mature (10, 12 and 15 mm from the tip) root zones, respectively, after 24 h of NaCl treatment (200 mM). Each measuring point is equivalent to the mean of at least 20 roots collected from 10 individual transgenic positive seedlings. (B) The histogram shows the average rate of net Na⁺ flux in the meristematic zone, elongation zone and mature zone. The error bar represents the standard error of the average value.