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. 2022 Jul 12;10(7):1406. doi: 10.3390/microorganisms10071406

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SAP11_PM19 binds but does not inhibit DNA binding activity of AtTCP19 in vitro. (a) SDS-PAGE of the recombinantly expressed and purified proteins MBP-AtTCP19-His (MBP-TCP19) and MBP-SAP11_PM19 (MBP-SAP11). M is a protein maker, lane 1 and 2 are MBP-SAP11_PM19 and MBP-AtTCP19-His, respectively. (b) Recombinant MBP-SAP11_PM19 binds to MBP-AtTCP19-His in an ELISA. The MBP-AtTCP19-His was coated on a microtiter plate and the MBP-SAP11_PM19 was added. The binding activity was detected using anti-SAP11_PM19 polyclonal antibody. BSA was used as a negative control. (c) EMSA agarose gel shows a band shift of C6-oligo-MBP-AtTCP19-His complex (lane 2). MBP-SAP11_PM19 cannot hinder the binding of MBP-AtTCP19-His to the C6-oligo, but form a super-shift band (lane 3). In lane 1 and 4, BSA and MBP-SAP11_PM19 cannot bind to C6-oligo-CY5 and are used as negative controls.