Figure 2.

Targeted-deletion and screening. (A) Twenty-four candidate mono-clones of 1527N were picked, followed by genomic DNA extraction and PCR analysis using a specific primer. The targeted-deletion obtained a 121 bp band. M, DL2000 DNA ladder. (B) Splicing-modulation efficiencies by targeting ISS-N1. (C) T-A cloning and sequencing of the positive clones. The deletions are indicated by a red dashed line. The column on the right indicates the percent of the relevant genotype in total sequencing reads. For 1527N-6, all the 20 reads were 9-nt deleted, which indicated 1527N-6 has three targeted-deletion copies. 1527N-18 has 14 reads with WT genotype and 9 reads with deleted 9-nt, indicating one targeted-deletion copy with two unedited copies. WT, wild-type. Δ, deletion.