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. 2022 Jul 19;23(14):7941. doi: 10.3390/ijms23147941

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differentiation of iPSCs into motor neurons. (A) Schematic protocol for motor neuron differentiation. NEPs, neuroepithelial progenitors; MNPs, motor neuron progenitors; iMNs, induced motor neurons; CHIR, CHIR99021; SB, SB431542; RA, retinal acid. (B) Dynamic changes in cellular morphology during differentiation of iPSC-derived MNs (iMNs). Scale bar: 500 μm. (C) Immunostaining of MNPs markers OLIG-2 at day 12 and motor neuron markers (ISL1, SMI32, HB9 at day 18, and ChAT, SMI32 at day 24). DAPI was used for nuclear staining. ISL1, ISLET1.