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. 2022 Jul 19;12(7):1744. doi: 10.3390/diagnostics12071744

Figure 8.

Figure 8

LAMP principle. The amplification starts when the FIP primer hybridizes to the F2c region of the strand. The F3 primer hybridizes to the F3c fragment on the DNA strand which initiates DNA synthesis by strand displacement. The strand bound to the FIP primer is then released and forms a loop structure at one end. This last structure allows the BIP primer to initiate subsequent DNA synthesis by strand displacement. This releases the sequence bound to BIP. The strand forms an altar-like structure and serves as the base for the LAMP cycles. The exponential amplification process then starts. BIP (or FIP) primer hybridizes to the loop structure. LF and LB primers are used to accelerate the amplification [135].