Table 1.

Characteristics of the reference RT-PCR and evaluated assays.

	Method	Volume of Sample **	Viral Genes Amplified (Number of Fluorphore)
			SARS-CoV-2	Influenza A	Influenza B	RSV
Alinity M RESP-4-Plex	RT-PCR	500 µL	ORF1b and N (1)	Matrix (1)	NS1 (1)	Matrix (1)
Idylla™ SARS-CoV-2	RT-PCR	200 µL	ORF1b (3) N (2)
Idylla™ SARS-CoV2/Flu/RSV	RT-PCR	400 µL	ORF1b (1) N (2)	- *	-	-
ID NOW COVID-19	Isothermal amplification	200 µL	ORF1b (1)
ID NOW influenza A & B 2	Isothermal amplification	200 µL		Matrix (1)	Matrix (1)	Fusion and Nucleocapsid (1)

* Pathogen detection not included in the assay. ** Recommended by the manufacturer.