Table 2.
Extracellular hydrolytic enzymes produced by the tested Actinomycetes isolates.
| Enzyme | Substrates | Staining | Diameter of Hydrolysis Area (mm) | ||
|---|---|---|---|---|---|
| AC1 Streptomyces sp. |
RS3 S. atratus |
OL2 A. humicola |
|||
| Chitinase | Chitin azure (1%) | Congo red (0.03%) | 23.0 ± 2.3 b | 0.0 ± 0.0 c | 31.5 ± 1.7 a |
| Chitin crab shells (1%) | Congo red (0.03%) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| Amylase | Soluble starch (1%) | Lugol solution (a) | 37.5 ± 2.9 a | 42.0 ± 1.2 a | 28.0 ± 3.5 b |
| Glucanase | Lichenan (0.2%) | Congo red (0.03%) | 22.0 ± 2.3 b | 0.0 ± 0.0 c | 36.0 ± 1.2 a |
| Pectinase | Pectin (0.5%) | CTAB (b) (2%) | 14.0 ± 1.2 a | 10.5 ± 1.7 a | 0.0 ± 0.00 b |
| Protease | Skim milk (1%) | - | 14.5 ± 2.9 b | 12.5 ± 2.9 b | 21.5 ± 1.7 a |
| Polygalacturanase | Polygalacturonic acid (1%) | Ruthenium red (0.1%) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
(a) Lugol solution was prepared as follows: 0.35 g iodide + 0.66 g potassium iodide KI in 100 mL dis. H2O; (b) CTAB: hexadecyltrimethylammonium bromide; values followed by different letters in each row for each tested enzyme were significantly different according to Tukey’s B multiple comparison test post hoc test at p < 0.05.