TABLE 4.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics and plasmid constructions | Reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| MFDpir | Donor strain containing the pSAM-Ec vector and auxotroph for diaminopimelate, ΔdapA::(erm-pir) | 56 |
| B. vietnamiensis | ||
| LMG10929 | Wild-type strain | 42 |
| Rifr | Spontaneous rifampicin resistance clone of LMG10929 | This study |
| Sptr | Spontaneous spectinomycin resistance clone of LMG10929 | This study |
| DsRed | LMG1029 + pIN29 | 6 |
| ΩcobW | Insertion mutant of pSHAFT2 in AK36_2246 | This study |
| ΩpdeR | Insertion mutant of pSHAFT2 in AK36_4666 | This study |
| Ωedd | Insertion mutant of pSHAFT2 in AK36_178 | This study |
| P. kururiensis | ||
| M130 | Wild-type strain | 9 |
| Rifr | Spontaneous rifampicin resistance clone of M130 | This study |
| Sptr | Spontaneous spectinomycin resistance clone of M130 | This study |
| DsRed | M130 + pIN29 | 6 |
| ΩcobW | Insertion mutant of pSHAFT2 in ANSKv1_10390 | This study |
| ΩpdeR | Insertion mutant of pSHAFT2 in ANSKv1_51226 | This study |
| Ωedd | Insertion mutant of pSHAFT2 in ANSKv1_70910 | This study |
| Plasmids | ||
| pIN29 | Carries genes conferring DsRed fluorescence and chloramphenicol resistance. DsRed is under control of the highly active pTAC promoter. | 55 |
| pSAM-Ec | Contains Himar1C9 transposase and a mariner transposon with a kanamycin resistance cassette | 57 |
| pSHAFT2 | Vector used for insertional mutagenesis containing a chloramphenicol resistance gene (nonreplicative plasmid) | 62 |