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. 2022 Jul 12;13:951318. doi: 10.3389/fpls.2022.951318

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Copyright © 2022 Wang, Wang, Zhang, Liu, Sun, Wang, Wang, Xing, Liu, Zhao, Shi, Su, Li, Xiao, Jiao, Li, Wang, Song and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression pattern and of Subcellular localization of RppM. (A) Expression analysis of RppM in Jing2416K roots, leaves, coleoptiles, and mesocotyls at the seedling stage and roots, first internodes, leaf sheaths, tassels, and ears at the mature stage was performed by quantitative real-time PCR. Data are means ± SD of three biological replicates. (B–E) The free GFP. (F–M) Subcellular localization of RppM protein in maize protoplasts. (F,J) RppM-GFP fusion protein. (C,G) Chloroplast autofluorenscence. (K) The nucleus marker D53-mCherry. (D,H,L) Bright field image. (E) Merged image of (B,C). (I) Merged image of (F,G). (M) Merged image of (F,G). Scale bar: 5 μm.