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. 2022 Jul 12;13:951318. doi: 10.3389/fpls.2022.951318

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Copyright © 2022 Wang, Wang, Zhang, Liu, Sun, Wang, Wang, Xing, Liu, Zhao, Shi, Su, Li, Xiao, Jiao, Li, Wang, Song and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Development of Kompetitive allele-specific PCR (KASP) markers for RppM and genotype analysis. (A) The two conserved SNPs and changes of amino acids in resistant and susceptible lines. SNP sites are indicated by red boxes. (B) Genotyping of inbred lines Jing2416K, Jing2416, Jing724, JingMC01, and Jing92 and F₁ plants of Jing2416 and Jing2416K with markers KM23 and KM19. Red, green, and blue dots represent susceptible, heterozygous resistant, and homozygous resistant genotypes, respectively. Black dots represent the non-template control (NTC). (C) Genotypes of F₂ plants derived from Jing2416 and Jing2416K. 1–10, resistant plants; 11–15, susceptible plants. (D) Thirteen inbred lines of a GWAS population identified with markers KM23 and KM19, all exhibiting SCR resistance.