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. 2022 Jul 13;14(14):3391. doi: 10.3390/cancers14143391

Figure 3.

Figure 3

Melanoma SC–CM-treated neutrophils exhibit increased production of both intracellular and extracellular ROS. (a) dHL60 cells were incubated with CSC–CM for 4 h. Intracellular ROS production was then measured by flow cytometry after staining with 2′,7′-dichlorofluorescin diacetate. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test. ** p < 0.01 vs. ctrl (control). (b) dHL60 cells were incubated with CSC–CM for 4 h. Extracellular ROS production was then measured by fluorescence analysis after staining with 2′,7′-dichlorofluorescin diacetate. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test. *** p < 0.001 vs. ctrl (control).