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. 2022 Jul 13;14(14):3391. doi: 10.3390/cancers14143391

Figure 5.

Figure 5

Melanoma SC-activated neutrophils secrete increased levels of tumor-promoting factors. (a) dHL60 cells were incubated with CSC-CM for 4 and 24 h, then their supernatant was changed with proper medium for 24 h. IL-8 secretion was then assessed by Elisa assay. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test. *** p < 0.001 vs. ctrl (control), **** p < 0.0001 vs. ctrl (control). (b) dHL60 cells were incubated with CSC–CM for 4 and 24 h, then their supernatant was changed with proper medium for 24 h. IL-6 secretion was then assessed by Elisa assay. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test. *** p < 0.001 vs. ctrl (control), **** p < 0.0001 vs. ctrl (control). (c) dHL60 cells were incubated with CSC–CM for 4 and 24 h, then their supernatant was changed with proper medium for 24 h. MMP-9 secretion was then assessed by Elisa assay. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test. *** p < 0.001 vs. ctrl (control), **** p < 0.0001 vs. ctrl (control). (d) dHL60 cells were incubated with CSC–CM for 4 and 24 h, then their supernatant was changed with proper medium for 24 h. TNF-α secretion was then assessed by Elisa assay. Each experiment was repeated three times. Data are expressed as mean values ± SEM and were analyzed by t-test.