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. 2022 Jul 13;14(14):3391. doi: 10.3390/cancers14143391

Figure 6.

Figure 6

Melanoma SC-incubated neutrophils confer stemness properties to melanoma non-stem cells. (a) A375 cells were incubated with dHL60–CM, obtained from dHL60 cells previously exposed to CSC–CM for 24 h. After 7 days, sphere formation assay was performed to determine the spheroidogenic ability of A375 cells. One representative experiment from three independent tests is shown. Scale bars are 30 μm. (b) A375 cells were incubated with dHL60–CM, obtained from dHL60 cells previously exposed to CSC–CM for 24 h. After 7 days, Western blot analysis was carried out to analyze the expression of ABCG2. GAPDH was used as a loading control. One representative experiment from three independent tests is shown.