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. 2022 Jul 12;13:896558. doi: 10.3389/fendo.2022.896558

Figure 5.

Figure 5

Effect of mitochondrial uncoupling on LPA-stimulating sperm motility. (A) The activity of mitochondria uncoupled by 10 µM CCCP (Ccc) was measured by 5 µM JC-1. Microscopic analysis showed the change of red/orange fluorescence of sperm treated with CCCP. (B) Spermatozoa were treated with 100 µM LPA (L) for 1 h in the absence or presence of 10 µM CCCP (Ccc), an uncoupler used to inhibit mitochondrial ATP production. The curvilinear velocity (VCL) of sperm motility was assessed by computer-assisted sperm analysis. Data are presented as mean ± SEM. n = 8. *p < 0.05 compared with the corresponding control (0 µM).