Table 1.
Effect of compactin on the zearalenone (ZEN) and deoxynivalenol (DON) production by F. culmorum FC-19 1.
| Compactin Concentration, μg/mL | Average Mycelium Dry Weight, mg | ZEN Production | DON Production | ||
|---|---|---|---|---|---|
| Average ZEN Content in the Culture Broth, μg | ZEN Production, μg/g of Dry Mycelium | Average DON Content in the Culture Broth, μg | DON Production, μg/g of Dry Mycelium | ||
| 0 (Control) | 661.67 ± 15.70 | 31.63 ± 4.01 | 47.74 ± 5.02 | 9.36 ± 0.67 | 14.17 ± 1.36 |
| ZEN experiment | |||||
| 25 | 612.67 ± 19.50 | 0.00 | 0.00 | - | - |
| DON experiment | |||||
| 10 | 650.00 ± 17.69 | - | - | 0.07 ± 0.07 | 0.11 ± 0.10 |
| 20 | 632.00 ± 11.79 | - | - | 0.00 | 0.00 |
| 50 | 406.01 ± 30.61 | - | - | 0.00 | 0.00 |
1 Measurements were made at the 7th day of the culture growth. The experiment was arranged in three replications; data are shown as M ± SD. LOD values for DON and ZEN were 2.0 and 0.5 ng/mL, respectively. Linear detection ranges for DON and ZEN were 5.0–400.0 and 2.0–250.0 ng/mL, respectively. The recovery for both toxins varied within 85–89%. The precision (%RSD) of HPLC measuring was 1.15 (DON) and 1.33 (ZEN).