Figure 3.

Undirected cytotoxicity of dianthin in the presence of the endosomal escape enhancer SO1861. Raji and Jurkat cells (20,000 cells/well) were seeded into a 96-well plate and allowed to grow for 24 h. They were then treated with (a) dianthin or (b) non-conjugated dianthin simply mixed with obinutuzumab. All proteins were applied at concentrations ranging from sub-femtomolar to upper nanomolar range. SO1861 was added at a final concentration of 1 µg/mL one hour before. Cells were incubated for 72 h in the presence of the compounds. Finally, cell proliferation was measured by an XTT assay and obtained values referred to untreated cells. Points in the graphs represent the mean ± SD of three biological experiments (n = 3) each one of them conducted in technical triplicate.