Figure 3.

Specific reconstitution of hnRNP I–vRNA binding restores wild-type growth kinetics in a mutant lacking the native interaction site. (A) Viral titers of wild-type and hnRNP–vRNA interaction site mutant viruses SINV.hnRNP I^TAR and SINV.hnRNP I^Δ at 24 h post-infection of mock and hnRNP I^TAT-transfected HEK293 cells infected at an MOI of 10 PFU per cell. (B) Comparative analysis of the viral titer for each of the aforementioned SINVs in mock-transfected HEK293 cells relative to wild-type SINV. (C) Identical to (B), with the exception that the HEK293 cells were transfected with hnRNP I^TAT. Quantitative data shown are the means of at least three minimum biological replicates, with the error bars representing the standard deviation of the means. Statistical significance, as determined by Student’s t-test, are indicated above the specific comparisons (with * ≤ 0.05; ** ≤ 0.01; *** ≤ 0.001).