Figure 5.

Viral RNA synthesis is not impacted by the hnRNP–vRNA interaction. Strand-specific quantitative analysis of the three SINV vRNA species in mock-transfected HEK293 cells infected with (A) SINV.WT or (B) SINV.hnRNP I^TAR viruses at an MOI of 10 PFU per cell. (C,D) Identical to the previously described panels, with the primary difference being that hnRNP I^TAT-transfected HEK293 cells were used. (E,F) Data from the previous panels reconfigured to allow direct comparisons between the viruses in either cell condition. Quantitative data shown are the means of three independent infections, with the error bars representing the standard deviation of the means. Statistical significance as determined by Student’s t-test is indicated above the specific comparisons (with * ≤ 0.05).