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. 2001 Oct;67(10):4414–4425. doi: 10.1128/AEM.67.10.4414-4425.2001

FIG. 1.

FIG. 1

ARDRA of distinct strains of the most abundant bacterial populations isolated from wheat rhizospheres in this study. All bacteria were cultured on 1/3× KMB+++. ARDRA of 16S sequences was performed using MspI and RsaI. Restriction digests of 16S sequences amplified from the most abundant cultured bacteria obtained from the 1998 (lanes 1 through 5) and 1999 (lanes 6 through 12) growth chamber experiments are shown, as are digests of sequences obtained from bacteria with the capacity to inhibit G. graminis var. tritici in vitro (lanes 13 through 15). The ARDRA group designation for each strain is indicated at the bottom of the figure. The five ARDRA groups identified in the1998 samples are represented by isolates U5 (lane 1), dI14 (lane 2), U3 (lane 3), dU1 (lane 4), and U1 (lane 5). The seven ARDRA groups identified in the 1999 samples are represented by strains C201A (lane 6), C201 (lane 7), C2+6 (lane 8), C2+4 (lane 9), E1+1 (lane 10), E102 (lane 11), and C204B (lane 12). In addition, representative strains with the ability to inhibit the take-all pathogen are also displayed: strain dI1 (lane 13), strain E206 (lane 14), and phlD+ strain MtV1 (lane 15). The sizes of individual fragments were determined based on the 100-bp ladder shown in lanes M.