FIG. 2.

Genotyping of phlD-containing bacteria present in the soils of Mount Vernon, Wash. Representative results for the growth chamber assays are shown. The phlD sequences were amplified using gene-specific primers B2BF and BPR4 and subsequently digested with either HaeIII or TaqI. Set 1 includes samples of several terminal phlD⁺ dilutions and isolates obtained from rhizospheres of wheat grown in soils A and B. Set 2 includes three isolates of a different genotype obtained from a Lind, Wash., soil for contrast. A known 2,4-DAPG-producing, BOX D genotype strain of Pseudomonas fluorescens (Q8r1–96) was used as a positive control for comparison (lane C). The sizes of individual fragments were determined based on the 100-bp ladder shown in lanes M.