TABLE 3.

Inhibition of G. graminis var. tritici and phlD⁺ strain MtV1 by representative isolates of the most abundant bacterial strains cultured from the rhizospheres of healthy and diseased wheat

Isolatea	phlDb	ARDRA groupc	Inhibition indexd for the following treatment at the indicated time:
			Isolate alone		Isolate plus MtV1e
			T1	T2	T1	T2
Q8r1	+	I	0.57 a	0.48 ab	0.58	0.50
MtVB	+	I	0.60 a	0.54 a	0.64	0.53
dI1	−	I	0.32 bc	0.26 c	0.43	0.36
dI1B	−	I	0.41 ab	0.29 bc	0.35	0.26
I1	−	I	0.35 abc	0.33 bc	0.34	0.29
I5B	−	I	0.35 abc	0.23 c	0.49	0.41
E206	−	I	0.34 ab	0.26 c	0.34	0.30
Other ARDRA group I, min	−	I	0.05 c	0.00 e	0.34	0.27
Other ARDRA group I, max	−	I	0.30 bc	0.08 e	0.59	0.55
ARDRA group II, min	−	II	0.07 bc	0.00 e	0.51	0.44
ARDRA group II, max	−	II	0.23 bc	0.07 e	0.63	0.58
All other, min	−	Other	0.00 c	0.00 e	0.46	0.39
All other, max	−	Other	0.29 bc	0.00 e	0.62	0.56

^aIsolates with significantly different inhibition phenotypes are grouped together for brevity, with the minimum (min) and maximum (max) inhibition indices for each genotype displayed. The total sample sizes were 22, 9, and 17 for other ARDRA group I, ARDRA group II, and all other isolates, respectively. 

^bPresence (+) or absence (−) of the phlD gene, a marker for 2,4-DAPG biosynthesis. 

^cGenotypes of isolates based on ARDRA of 16S sequences with MspI and RsaI. 

^dInhibition indices range from 0 (no inhibition) to 1 (total inhibition of fungal growth). Measurements were taken at two different time points, T1 (where uninhibited fungal growth extended 50 to 75% of the radius of the assay plate) and T2 (where uninhibited fungal growth extended the full radius of the assay plate). Values followed by different letters are significantly different, as determined by the Tukey-Kramer multiple-range test (P < 0.10). 

^eTested strains were mixed 3:1 with strain MtV1 prior to inoculation on the assay plates.